Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 157, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2022.113116
Keywords
Succinyllation; Milk proteins; Protein-zinc complex; Zinc holding ability; Processing conditions
Categories
Funding
- Ministry of Science and Technology, New Delhi, India [SERB/MOFPI/0040/2013]
Ask authors/readers for more resources
The research evaluated the interaction of zinc with different forms of milk proteins, finding that succinylation can enhance the binding ability of milk proteins. The diafiltration process can optimize milk protein-Zinc complexes, which could be used for food fortification to prevent zinc deficiency.
Milk proteins with substantial reactive groups enable new protein chemistries and succinylation being a potential post-translational modification plays a significant role in regulating milk protein configuration properties. The presence of additional carboxylic group due to succinylation also improves the divalent metal binding efficiency of milk proteins. Therefore, in the present research work, the interaction of zinc (Zn) with native and succinylated milk protein concentrate (MPC) and sodium caseinate (NaCN) was assessed. For the optimization of milk protein-Zn complexes, a diafiltration process was adopted and this process efficiently removed the free form of succinic acid which was confirmed by scanning electron microscopy. Maximum Zn binding ability of MPC and succinylated MPC (SMPC) was observed at 2.0 and 7.0 mmol/L Zn concentration, whereas NaCN and SNaCN showed maximum Zn binding ability at 2.4 and 7.6 mmol/L Zn concentrations, respectively. Processing variables such as pH and thermal treatment did not alter the Zn holding capacity of SMPC and SNaCN, however, ionic concentration >= 0.3 mol/L NaCl significantly decreased (P < 0.05) it. Therefore, based on the key findings, the milk protein-Zn complex can be utilized for the fortification of various food products to eradicate Zn deficiency.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available