4.5 Article

Application of 2,4,5-tris(2-pyridyl)imidazole as 'turn-off' fluorescence sensor for Cu(II) and Hg(II) ions and in vitro cell imaging

Journal

LUMINESCENCE
Volume 37, Issue 6, Pages 883-891

Publisher

WILEY
DOI: 10.1002/bio.4232

Keywords

'turn-off' sensor; cell imaging; fluorescence; heterocycle; imidazole

Funding

  1. Ministry of Education (MoE)
  2. Department of Biotechnology (DBT)
  3. North East Centre for Biological Sciences and Healthcare Engineering (NECBH)
  4. Central Instrument Facility (CIF)
  5. Fund for Improvement in Science and Technology (FIST)
  6. high-resolution mass spectrometry (HRMS)
  7. Department of Science and Technology (DST)

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The molecule 2,4,5-tris(2-pyridyl)imidazole (L) has been evaluated as a probe for dual sensing of Hg2+ and Cu2+ ions. It shows a sensitive and selective response in the presence of both ions and can detect them in specific pH ranges. The probe's detection limit for Cu2+ is below the allowable limit prescribed by the United States Environmental Protection Agency. Experimental observations are supported by calculations and cell imaging studies.
The 2,4,5-tris(2-pyridyl)imidazole (L) molecule has been evaluated as a probe for dual sensing of Hg2+ and Cu2+ ions in EtOH/HEPES buffer medium (5 mM, pH = 7.34, 1:1, v/v). Probe L shows a good sensitive and selective turn-off response in the presence of both Hg2+ and Cu2+ ions, which is comprehensible under long UV light. The probe can detect Cu2+ ion in the pH range 3-11 and Hg2+ ion in pH 6-8. The limit of detection for Cu2+ (0.77 mu M) is well under the allowable limit prescribed by the United States Environmental Protection Agency. Two metal (Cu2+/Hg2+) ions are needed per L for complete fluorescence quenching. The probe shows marked reversibility on treatment with Na(2)EDTA, making the protocol more economical for practical purposes. Paper strip coated with the L solution of EtOH can detect the presence of Cu2+ and Hg2+ ions in the sample using visible quenching of the fluorescence intensity. Density functional theory-time-dependent density functional theory (DFT-TDDFT) calculations support experimental observations, and d-orbitals of Cu2+/Hg2+ provide a nonradiative decay pathway. Cell imaging study using HDF and MDA-MB-231 cells also supported the viability of L in detecting Cu2+ and Hg2+ ions in living cells.

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