Journal
LUMINESCENCE
Volume 37, Issue 6, Pages 883-891Publisher
WILEY
DOI: 10.1002/bio.4232
Keywords
'turn-off' sensor; cell imaging; fluorescence; heterocycle; imidazole
Categories
Funding
- Ministry of Education (MoE)
- Department of Biotechnology (DBT)
- North East Centre for Biological Sciences and Healthcare Engineering (NECBH)
- Central Instrument Facility (CIF)
- Fund for Improvement in Science and Technology (FIST)
- high-resolution mass spectrometry (HRMS)
- Department of Science and Technology (DST)
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The molecule 2,4,5-tris(2-pyridyl)imidazole (L) has been evaluated as a probe for dual sensing of Hg2+ and Cu2+ ions. It shows a sensitive and selective response in the presence of both ions and can detect them in specific pH ranges. The probe's detection limit for Cu2+ is below the allowable limit prescribed by the United States Environmental Protection Agency. Experimental observations are supported by calculations and cell imaging studies.
The 2,4,5-tris(2-pyridyl)imidazole (L) molecule has been evaluated as a probe for dual sensing of Hg2+ and Cu2+ ions in EtOH/HEPES buffer medium (5 mM, pH = 7.34, 1:1, v/v). Probe L shows a good sensitive and selective turn-off response in the presence of both Hg2+ and Cu2+ ions, which is comprehensible under long UV light. The probe can detect Cu2+ ion in the pH range 3-11 and Hg2+ ion in pH 6-8. The limit of detection for Cu2+ (0.77 mu M) is well under the allowable limit prescribed by the United States Environmental Protection Agency. Two metal (Cu2+/Hg2+) ions are needed per L for complete fluorescence quenching. The probe shows marked reversibility on treatment with Na(2)EDTA, making the protocol more economical for practical purposes. Paper strip coated with the L solution of EtOH can detect the presence of Cu2+ and Hg2+ ions in the sample using visible quenching of the fluorescence intensity. Density functional theory-time-dependent density functional theory (DFT-TDDFT) calculations support experimental observations, and d-orbitals of Cu2+/Hg2+ provide a nonradiative decay pathway. Cell imaging study using HDF and MDA-MB-231 cells also supported the viability of L in detecting Cu2+ and Hg2+ ions in living cells.
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