Journal
LUMINESCENCE
Volume 37, Issue 6, Pages 987-994Publisher
WILEY
DOI: 10.1002/bio.4250
Keywords
antibiotics; aptamer; AuNPs; chemiluminescence; recycling
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Funding
- Laoshan Scholar Programme of Qingdao University of Science and Technology [201802685]
- Open Foundation from the Academic Division of Chemistry, Qingdao University of Science and Technology [QUSTHX201907]
- National Natural Science Foundation of China [21575073]
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A chemiluminescence (CL) sensing strategy for kanamycin residue detection in fish samples was developed using luminol-functionalized gold nanoparticles as CL nanoprobe materials, combined with DNA hairpin structure and carboxyl-modified magnetic beads. This method showed excellent sensitivity and a good linear relationship, providing a low-cost and high-sensitivity CL analysis strategy.
A chemiluminescence (CL) sensing strategy for kanamycin residue detection in fish samples was established based on luminol-functionalized gold nanoparticles as CL nanoprobe materials combined with DNA hairpin structure and carboxyl-modified magnetic beads. Relying on nucleic acid amplification technology, the system can successfully realize the recycling of kanamycin, so that the biosensor can release a large number of luminol-functionalized gold nanoparticles with excellent CL performance even at a low residual levels of kanamycin. The biosensor strategy showed a good linear relationship with kanamycin in the range 0.09-130 nM, the detection limit was as low as 0.04 nM. This method proves the excellent performance of the sensing strategy and provides a low-cost and high-sensitivity CL analysis strategy for the detection of kanamycin and even other antibiotics.
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