Journal
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
Volume 169, Issue 5, Pages -Publisher
ELECTROCHEMICAL SOC INC
DOI: 10.1149/1945-7111/ac6c55
Keywords
nanopore; polyelectrolytes; Alpha-Hemolysin; Mycobacterium smegmatis Porin A
Funding
- Biomedical Engineering Program, University of South Carolina
- Department of Chemical Engineering, University of South Carolina
- National Institute of Allergy and Infectious Diseases (NIAID) [K22AI136686]
- South Carolina IDeA Networks of Biomedical Research Excellence Developmental Research Project by the National Institute of General Medical Sciences (NIGMS) [P20GM103499]
- National Science Foundation (NSF) CAREER Award [2047503]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [2047503] Funding Source: National Science Foundation
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In this study, the potential of diblock synthetic polyelectrolytes as nanopore sensing probes was explored by investigating their translocation behaviors through alpha-hemolysin and MspA nanopores. The results indicate efficient translocations of PEO-b-PSS through alpha-HL and PEO-b-PVBTMA through MspA, suggesting that synthetic polyelectrolytes can offer more flexibility and cost-effectiveness for nanopore sensing.
DNAs have been used as probes for nanopore sensing of noncharged biomacromolecules due to its negative phosphate backbone. Inspired by this, we explored the potential of diblock synthetic polyelectrolytes as more flexible and inexpensive nanopore sensing probes by investigating translocation behaviors of PEO-b-PSS and PEO-b-PVBTMA through commonly used alpha-hemolysin (alpha-HL) and Mycobacterium smegmatis porin A (MspA) nanopores. Translocation recordings in different configurations of pore orientation and testing voltage indicated efficient PEO-b-PSS translocations through alpha-HL and PEO-b-PVBTMA translocations through MspA. This work provides insight into synthetic polyelectrolyte-based probes to expand probe selection and flexibility for nanopore sensing.
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