Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 33, Issue 4, Pages 660-670Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jasms.1c00334
Keywords
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Funding
- Natural Sciences and Engineering Research Council (NSERC) [RGPIN 2016-0470, STPGP 401252850]
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The rapid calibration chip (RCC) uses the wetting behavior of hydrophilic/hydrophobic patterns to confine droplets and obtain a calibration curve. It doesn't require advanced equipment and can form multiple droplet series quickly. Evaluation of its performance allows for determining liquid volumes.
The rapid calibration chip (RCC) is a device that uses the fast and reproducible wetting behavior of hydrophilic/ hydrophobic patterned surfaces to confine a series of differently sized droplets on a substrate to obtain a calibration curve. Multiple series of droplets can be formed within seconds by dipping an RCC into a calibration solution. No pipetting, sequential droplet deposition, or advanced equipment is required. The performance and reproducibility of RCCs were evaluated with an electrospray ionization triple-quadrupole mass spectrometer equipped with a liquid microjunction-surface sampling probe (LMJ-SSP) that allows for fast sampling of surfaces. Using circular hydrophilic areas with diameters ranging from 0.25 to 2.00 mm, liquid volumes of 4.6-70.6 nL could be deposited. Furthermore, the use of a second hydrophobic/hydrophilic patterned transfer chip can be used to add internal standard solutions to each calibration spot of the RCC, allowing to transfer a liquid volume of 22.5 nL.
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