Screening of hepatitis B virus DNA in the serum sample by a new sensitive electrochemical genosensor-based Pd-Al LDH substrate

Screening for hepatitis B is one of the most severe liver virus infections in the world, and due to the effect of early detection and treatment of hepatitis B disease, fast and sensitive analytical devices are highly needed. A highly sensitive voltammetric genosensor is described for hepatitis B virus (HBV) detection by depositing electrochemically Pd(II)-Al(III)-layered double hydroxide (LDH) in a short time by chronoamperometry (CA) technique on the surface of the fluorinated tin oxide electrode (FTO) that is reported for the first time. The complementary DNA sequence of the amino-labeled DNA was fixed as a probe on the carboxylic group-directed LDH film. The electrochemical behavior of the designed genosensor by the attached DNA virus was investigated using the differential pulse voltammetry (DPV), cyclic voltammetric (CV), and electrochemical impedance spectroscopy (EIS) techniques in the presence of the Fe(CN)(6)(3-/4-) and at the optimum conditions has a wide linear range (1 fM to 1 mu M) with the experimental detection limit of 1 fM. This genosensor was successfully implemented to measure the DNA of HBV in human serum. This modified electrode has high sensitivity and short production time, which can be a candidate for the production of a portable hepatitis B diagnostic kit.

Automated summary

This article describes a highly sensitive voltammetric genosensor for the detection of hepatitis B virus (HBV) by depositing Pd(II)-Al(III)-layered double hydroxide (LDH) on the surface of a fluorinated tin oxide electrode (FTO) using chronoamperometry (CA) technique. The genosensor exhibits high sensitivity, a wide linear range, and a low detection limit, and has been successfully applied for the measurement of HBV DNA in human serum.