1H NMR and chemometric methods in verification of hemp-seed oil quality

Hemp oil is widely used in the food industry, and also in many other industries. It is characterized by a high content of unsaturated fatty acids with the optimal ratio of omega-6 to omega-3 fatty acids (3:1). Such composition means that this oil is often used in the food, pharmaceutical and cosmetic industries. Due to the popularity of hemp oil and its high content of unsaturated fatty acids, methods for the quick oil quality analysis are sought. Six samples of cold-pressed hemp oil were stored under different conditions (2-8 degrees C and 30 degrees C) for one month and then their H-1 NMR spectra were recorded. The chemometric methods of PCA and OPLS-DA were used to perform the analysis of the spectra. The combination of those methods made it possible to observe the differences in the oil samples after a month of storage. Based on the H-1 NMR spectra, it was possible to differentiate the time and conditions of the oil storage.

Automated summary

Hemp oil, with its high content of unsaturated fatty acids, is widely used in the food, pharmaceutical, and cosmetic industries. The analysis of H-1 NMR spectra reveals the differences in oil samples after different storage times and conditions.