4.7 Review

Insights gained in the pathology of lung disease through single-cell transcriptomics

Related references

Note: Only part of the references are listed.
Article Cell Biology

A census of the lung: CellCards from LungMAP

Xin Sun et al.

Summary: The human lung plays important roles in respiration, host defense, and basic physiology. Recent technological advancements have led to the discovery of new cell types and functional properties in the lung. A comprehensive cellular census of the lung has been synthesized, with detailed information on cell types, function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication aims to serve as a guide for lung research and promote efforts to establish, maintain, and restore respiratory health.

DEVELOPMENTAL CELL (2022)

Article Cell Biology

Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells

Jaymin J. Kathiriya et al.

Summary: The study reveals that human alveolar type 2 cells can transdifferentiate into basal cells in response to fibrotic signaling, driving the pathology associated with severe lung injuries. Aberrant mesenchyme plays a crucial role in the lineage trajectory leading to the expansion of alveolar metaplastic basal cells.

NATURE CELL BIOLOGY (2022)

Article Multidisciplinary Sciences

Cellular and molecular architecture of submucosal glands in wild-type and cystic fibrosis pigs

Wenjie Yu et al.

Summary: Submucosal glands (SMGs) play a crucial role in protecting the lungs but can also contribute to disease. In cystic fibrosis (CF), SMGs produce abnormal mucus that disrupts mucociliary transport. This study used single-cell RNA sequencing, immunohistochemistry, and in situ hybridization to investigate the expression and distribution of ion transporters and cell types in SMGs. The results suggest that loss of epithelial anion secretion, rather than intrinsic cell defects, causes CF mucus abnormalities. The study also revealed different functions of acinar mucous cells and acinar serous cells in producing mucins and antimicrobials, respectively.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2022)

Article Multidisciplinary Sciences

Human distal lung maps and lineage hierarchies reveal a bipotent progenitor

Preetish Kadur Lakshminarasimha Murthy et al.

Summary: This study identifies previously unknown cell types in the human distal airways and reveals their roles in lung development and regeneration. These findings are important for understanding the mechanisms underlying respiratory diseases.

NATURE (2022)

Article Multidisciplinary Sciences

Human distal airways contain a multipotent secretory cell that can regenerate alveoli

Maria C. Basil et al.

Summary: This study reveals the existence of a unique population of secretory cells in the respiratory bronchioles of the human lung, which act as unidirectional progenitors for alveolar type 2 cells. The differentiation of these respiratory airway secretory cells into alveolar type 2 cells is regulated by Notch and Wnt signaling. These findings highlight the importance of this distinct progenitor cell population in maintaining the gas-exchange compartment and its potential role in chronic lung diseases.

NATURE (2022)

Article Critical Care Medicine

Senescence of Alveolar Type 2 Cells Drives Progressive Pulmonary Fibrosis

Changfu Yao et al.

Summary: The study demonstrates that senescence of AT2 cells is sufficient to drive progressive pulmonary fibrosis, and interventions targeting p53 activation or senescence can block fibrogenesis. Early attenuation of senescence-related pathways and elimination of senescent cells are promising therapeutic approaches to prevent pulmonary fibrosis.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2021)

Article Critical Care Medicine

Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia

Kenichi Okuda et al.

Summary: The study revealed that secretory cells dominate CFTR expression and function in human airway superficial epithelia, suggesting that CFTR therapies may need to restore CFTR function to multiple cell types, with a focus on secretory cells.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2021)

Editorial Material Biotechnology & Applied Microbiology

Gene Therapy for Cystic Fibrosis: Lessons Learned and Paths Forward

Soon H. Choi et al.

MOLECULAR THERAPY (2021)

Correction Biochemistry & Molecular Biology

The basic immunology of asthma (vol 184, pg 1469, 2021)

Hamida Hammad et al.

Article Biochemistry & Molecular Biology

Transcriptional analysis of cystic fibrosis airways at single-cell resolution reveals altered epithelial cell states and composition

Gianni Carraro et al.

Summary: Single-cell RNA profiling of human cystic fibrosis proximal airway tissue shows an abundance of epithelial cells transitioning to specialized ciliated and secretory cells, coupled with a decrease in cycling basal cells. The study provides insights into disease-related changes and offers potential for the development of new targeted therapies for cystic fibrosis airway disease.

NATURE MEDICINE (2021)

Article Respiratory System

Age-specific incidence of allergic and non-allergic asthma

Johanna Pakkasela et al.

BMC PULMONARY MEDICINE (2020)

Article Biochemistry & Molecular Biology

Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis

Wei Rao et al.

Article Critical Care Medicine

Single-Cell Reconstruction of Human Basal Cell Diversity in Normal and Idiopathic Pulmonary Fibrosis Lungs

Gianni Carraro et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2020)

Article Critical Care Medicine

A Single-Cell Atlas of the Human Healthy Airways

Marie Deprez et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2020)

Editorial Material Physiology

The Idiopathic Pulmonary Fibrosis Cell Atlas

Nir Neumark et al.

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY (2020)

Article Multidisciplinary Sciences

Alveolar regeneration through a Krt8+transitional stem cell state that persists in human lung fibrosis

Maximilian Strunz et al.

NATURE COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

A molecular cell atlas of the human lung from single-cell RNA sequencing

Kyle J. Travaglini et al.

NATURE (2020)

Review Medicine, General & Internal

Diagnosis and Outpatient Management of Chronic Obstructive Pulmonary Disease A Review

Craig M. Riley et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2019)

Review Critical Care Medicine

Lung transplantation for idiopathic pulmonary fibrosis

Peter M. George et al.

LANCET RESPIRATORY MEDICINE (2019)

Review Immunology

The Cytokines of Asthma

Bart N. Lambrecht et al.

IMMUNITY (2019)

Article Respiratory System

Proliferating SPP1/MERTK-expressing macrophages in idiopathic pulmonary fibrosis

Christina Morse et al.

EUROPEAN RESPIRATORY JOURNAL (2019)

Article Biochemistry & Molecular Biology

A cellular census of human lungs identifies novel cell states in health and in asthma

Felipe A. Vieira Braga et al.

NATURE MEDICINE (2019)

Review Medicine, General & Internal

Update on the Pathogenesis of Chronic Obstructive Pulmonary Disease

Alvar Agusti et al.

NEW ENGLAND JOURNAL OF MEDICINE (2019)

Article Medicine, General & Internal

Elexacaftor-Tezacaftor-Ivacaftor for Cystic Fibrosis with a Single Phe508del Allele

P. G. Middleton et al.

NEW ENGLAND JOURNAL OF MEDICINE (2019)

Article Critical Care Medicine

Single-Cell Transcriptomic Analysis of Human Lung Provides Insights into the Pathobiology of Pulmonary Fibrosis

Paul A. Reyfman et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2019)

Article Critical Care Medicine

Multiview Cluster Analysis Identifies Variable Corticosteroid Response Phenotypes in Severe Asthma

Wei Wu et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2019)

Review Medicine, General & Internal

Asthma

Alberto Papi et al.

LANCET (2018)

Review Medicine, General & Internal

Idiopathic Pulmonary Fibrosis

David J. Lederer et al.

NEW ENGLAND JOURNAL OF MEDICINE (2018)

Editorial Material Critical Care Medicine

COPD beyond smoking: new paradigm, novel opportunities

Alvar Agusti et al.

LANCET RESPIRATORY MEDICINE (2018)

Article Multidisciplinary Sciences

A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte

Lindsey W. Plasschaert et al.

NATURE (2018)

Article Multidisciplinary Sciences

A revised airway epithelial hierarchy includes CFTR-expressing ionocytes

Daniel T. Montoro et al.

NATURE (2018)

Article Respiratory System

COPD: time to improve its taxonomy?

Bartolome R. Celli et al.

ERJ OPEN RESEARCH (2018)

Article Immunology

The immunology of the allergy epidemic and the hygiene hypothesis

Bart N. Lambrecht et al.

NATURE IMMUNOLOGY (2017)

Article Respiratory System

Metabolomic biomarkers predictive of early structural lung disease in cystic fibrosis

Charles R. Esther et al.

EUROPEAN RESPIRATORY JOURNAL (2016)

Article Allergy

Inflammatory mechanisms in patients with chronic obstructive pulmonary disease

Peter J. Barnes

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY (2016)

Review Respiratory System

Bronchial epithelium in children: a key player in asthma

Ania Carsin et al.

EUROPEAN RESPIRATORY REVIEW (2016)

Review Medicine, General & Internal

Origins of Cystic Fibrosis Lung Disease

David A. Stoltz et al.

NEW ENGLAND JOURNAL OF MEDICINE (2015)

Article Immunology

Type 2 inflammation in asthma - present in most, absent in many

John V. Fahy

NATURE REVIEWS IMMUNOLOGY (2015)

Article Critical Care Medicine

Revealing the Pathogenic and Aging-related Mechanisms of the Enigmatic Idiopathic Pulmonary Fibrosis An Integral Model

Moises Selman et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2014)

Article Biochemistry & Molecular Biology

Lung Phenotype of Juvenile and Adult Cystic Fibrosis Transmembrane Conductance Regulator-Knockout Ferrets

Xingshen Sun et al.

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY (2014)

Article Medicine, General & Internal

A Phase 3 Trial of Pirfenidone in Patients with Idiopathic Pulmonary Fibrosis

Talmadge E. King et al.

NEW ENGLAND JOURNAL OF MEDICINE (2014)

Article Medicine, General & Internal

Efficacy and Safety of Nintedanib in Idiopathic Pulmonary Fibrosis

Luca Richeldi et al.

NEW ENGLAND JOURNAL OF MEDICINE (2014)

Review Biochemistry & Molecular Biology

Animal Models of Fibrotic Lung Disease

Bethany B. Moore et al.

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY (2013)

Article Medicine, General & Internal

Idiopathic pulmonary fibrosis

Talmadge E. King et al.

LANCET (2011)

Article Cell Biology

Cystic Fibrosis Pigs Develop Lung Disease and Exhibit Defective Bacterial Eradication at Birth

David A. Stoltz et al.

SCIENCE TRANSLATIONAL MEDICINE (2010)

Review Medicine, General & Internal

Chronic obstructive pulmonary disease in non-smokers

Sundeep S. Salvi et al.

LANCET (2009)

Article Multidisciplinary Sciences

Short telomeres are a risk factor for idiopathic pulmonary fibrosis

Jonathan K. Alder et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

Article Biotechnology & Applied Microbiology

Repeated aerosolized AAV-CFTR for treatment of cystic fibrosis: A Randomized placebo-controlled phase 2B trial

Richard B. Moss et al.

HUMAN GENE THERAPY (2007)

Article Biochemistry & Molecular Biology

Apoptosis and emphysema - The missing link

RM Tuder et al.

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY (2003)

Article Public, Environmental & Occupational Health

Occupational and environmental risk factors for idiopathic pulmonary fibrosis: A multicenter case-control study

KB Baumgartner et al.

AMERICAN JOURNAL OF EPIDEMIOLOGY (2000)

Review Medicine, General & Internal

Medical progress: Chronic obstructive pulmonary disease.

PJ Barnes

NEW ENGLAND JOURNAL OF MEDICINE (2000)

Article Medicine, Research & Experimental

Endosomal processing limits gene transfer to polarized airway epithelia by adeno-associated virus

DS Duan et al.

JOURNAL OF CLINICAL INVESTIGATION (2000)