Journal
JOURNAL OF MOLECULAR STRUCTURE
Volume 1252, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.molstruc.2021.132185
Keywords
Ginkgo biloba leaves; Polysaccharides; Structure; Antioxidant activity
Categories
Funding
- National Natural Science Foundation of China [32171836, 31770725]
- Natural Science Foundation of Liaoning Province [2021-MS-299, 2020-MZLH-37, LJKZ0538]
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Ginkgo biloba leaves polysaccharide is considered as an important functional factor with an acidic polysaccharide structure, containing various glycosidic linkages, and showing strong antioxidant activities.
Ginkgo biloba extract is regarded as one of the most popular natural plant preparations, among which polysaccharide is considered to be an important functional factor. In the study, polysaccharide was extracted from Ginkgo biloba leaves powder by ultrasonic-enzyme assisted water-soluble alcohol precipitation. The content of crude polysaccharide was 82.4% by the phenol sulfuric acid method. Structure and chemical composition of Ginkgo biloba leaves polysaccharide were investigated by chromatographic and nuclear magnetic resonance spectoscocopy analyses. Results showed that the purified Ginkgo biloba leaves polysaccharide was an acidic polysaccharide with a molecular weight of 12.08 kDa, containing glycosidic linkages of beta-D-Rhap-(1 ->, beta-L-Xylp-(1 ->, -> 4)-beta-D-Manp-(1 ->, -> 4)- ss-D-Glcp-(1 ->, -> 6)-beta-D-Manp-(1 ->, -> 3)-beta-L-Araf-(1 ->, -> 4)-beta-D-Galp-(1 ->, -> 3,4)-beta-D-Glcp-(1 ->. The monosaccharides were composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 5.56:3.84:2.34:7.54:8.34:1, respectively. Scanning electron microscopy analysis showed that the microscopic conformation of the purified Ginkgo biloba leaves polysaccharide was fluffy and irregular honeycomb with certain pores. Antioxidant activities in vitro, including scavenging 1,1-Diphenyl-2-picrylhydrazine, scavenging hydroxyl radical activity and scavenging superoxide anion were 78.8%, 79.2% and 43.7%, respectively, which suggested that the purified Ginkgo biloba leaves polysaccharide had strong antioxidant activities. These findings indicate that Ginkgo. biloba leaves polysaccharides have potential as natural antioxidants in food processing. (C) 2021 Elsevier B.V. All rights reserved.
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