4.3 Article

Real-time quantitative PCR method for detection and quantification of Clostridioides difficile cells and spores

Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 196, Issue -, Pages -

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ELSEVIER
DOI: 10.1016/j.mimet.2022.106458

Keywords

Clostridioides difficile; qPCR; Pathogen detection; Spores

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A quantitative PCR method has been developed to detect the Chaperonin-60 gene of Clostridioides difficile. This method is specific to C. difficile strains of clinical and epidemiological importance and enables the quantification of C. difficile cells and spores.
A quantitative PCR method was developed for the detection of Clostridioides difficile Chaperonin-60 gene. The method had a specificity for C. difficile strains of clinical and epidemiological importance and allowed for quantification of C. difficile cells and spores.

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