Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 195, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2022.106454
Keywords
Corynebacterium ulcerans; Corynebacterium pseudotuberculosis; Corynebacterium diphtheriae; Diphtheria toxin; Multiplex real -time PCR
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Funding
- Japan Agency for Medical Research and Development [JP20fk0108097, JP21fk0108097]
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A multiplex real-time PCR assay has been developed to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay is a valuable tool for identification of potentially toxigenic C. ulcerans.
We developed a multiplex real-time PCR assay with amplicon melting curve analysis to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay should be a valuable tool for identification of potentially toxigenic C. ulcerans.
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