Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 112, Issue 1, Pages 157-172Publisher
OXFORD UNIV PRESS
DOI: 10.1002/JLB.4MA0222-419RR
Keywords
adaptive immunity; bone marrow; cross-presentation; ubiquitination; Cytotoxicity; T cells; Cross-presentation; CTL
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Funding
- National Natural Science Foundation of China [81273203, 81771669]
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K48 and K27-linked ubiquitination play vital roles in cross-presentation and BMPC-based adaptive immunity by affecting BMPC cross-presentation and cytolytic capacity in BMPC-activated T cells.
K48-linked ubiquitination determines antigen degradation and plays vital roles in the process of cross-presentation of bone marrow precursor cell (BMPC)-derived dendritic cells (DCs). Although previous studies revealed that K48 and K27-linked ubiquitination regulates innate immunity, the exact roles of K48 and K27-linked ubiquitination in cross-presentation and BMPC-based adaptive immunity are still uncertain. In this study, we investigated the effects of K48- and K27-mutant ubiquitin (Ub) on BMPC-based adaptive immune response by observing the effects of MG132, Ub deficiency, and K48/K27-mutant Ub on cross-presentation, T cell proliferation, IFN-gamma secretion, BMPC-based CTL priming, and thereby the efficiency of cytolytic capacity of BMPC-activate T cells. We demonstrated that MG132, Ub deficiency, and K48-mutant Ub impair cross-presentation, T cell proliferation, IFN-gamma secretion, BMPC-based CTL priming, and the cytolytic capacity of BMPC-activated T cells. Moreover, although K27-only Ub decreases cross-presentation, the replenishment of K27-mutant Ub restores Ub deficiency impaireds the abilities of T cell proliferation, IFN-gamma secretion, CTL priming, and the cytolytic capacity of BMPC-activated T cells. Thus, these data suggest that K48- and K27-linked ubiquitination contributes to BMPC-mediated adaptive immune response by affecting BMPC cross-presentation and the cytolytic capacity by up-regulating both perforin and granzyme B in BMPC-activated T cells. K48- and K27-mutant Ub might have potential clinical therapeutic function in adaptive immune response-associated diseases.
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