Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 226, Issue 5, Pages 766-777Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiac091
Keywords
SARS-CoV-2; COVID-19; complement system; classical pathway; common cold coronaviruses; and antibodies
Categories
Funding
- University of Pittsburgh Clinical and Translational Science Institute
- DSF Charitable Foundation
- National Heart, Lung, and Blood Institute, National Institutes of Health [139987]
- University of Pittsburgh Medical Center
- National Institute of Allergy and Infectious Diseases (Pittsburgh AIDS Research Training Fellowship-National Research Service Award) [5T32AI065380-15]
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This study reveals that the classical pathway mediates complement overactivation in response to increased levels of circulating immune complexes, leading to the severity of COVID-19. Early non-neutralizing IgG responses play a crucial role in complement overactivation.
Our findings reveal that complement overactivation is mediated by the classical pathway in response to increased levels of circulating immune complexes and support the notion that an overexuberant immunoglobulin G response against severe acute respiratory syndrome coronavirus 2 and seasonal coronaviruses contributes to coronavirus disease 2019 severity. Background Excessive complement activation has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19), but the mechanisms leading to this response remain unclear. Methods We measured plasma levels of key complement markers, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and antibodies against SARS-CoV-2 and seasonal human common cold coronaviruses (CCCs) in hospitalized patients with COVID-19 of moderate (n = 18) and critical severity (n = 37) and in healthy controls (n = 10). Results We confirmed that complement activation is systemically increased in patients with COVID-19 and is associated with a worse disease outcome. We showed that plasma levels of C1q and circulating immune complexes were markedly increased in patients with severe COVID-19 and correlated with higher immunoglobulin (Ig) G titers, greater complement activation, and higher disease severity score. Additional analyses showed that the classical pathway was the main arm responsible for augmented complement activation in severe patients. In addition, we demonstrated that a rapid IgG response to SARS-CoV-2 and an anamnestic IgG response to the nucleoprotein of the CCCs were strongly correlated with circulating immune complex levels, complement activation, and disease severity. Conclusions These findings indicate that early, nonneutralizing IgG responses may play a key role in complement overactivation in severe COVID-19. Our work underscores the urgent need to develop therapeutic strategies to modify complement overactivation in patients with COVID-19.
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