Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 505, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jim.2022.113275
Keywords
ELISA; COVID-19 vaccine; Peptide-based vaccine; SARS-CoV-2; Golden Syrian hamsters
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Funding
- Mayo Clinic
- ICW Ventures for preclinical studies on a peptide-based COVID-19 vaccine
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This paper describes the development of an ELISA method for detecting antibodies specific to peptides and proteins derived from the SARS-CoV-2 virus in immunized golden Syrian hamsters. The method allows quantitative assessment of humoral immune responses at the peptide and protein levels, and has potential applications in the development and testing of peptide-based vaccines in hamsters.
Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.
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