The role and its transcriptome mechanisms of cell wall polysaccharides in vanadium detoxication of rice

Cell wall-polysaccharides play a crucial role in heavy metals binding, and hence, contribute to heavy metal detoxication in plants. However, there is no data regarding the molecular mechanisms of vanadium (V) binding to root cell walls in plants, especially in rice (Oryza sativa L.). Taking two rice cultivars with various V tolerance as the research material, the present study investigated the effect of various V concentrations on subcellular distribution of V and revealed the regulatory mechanism of cell wall polysaccharides to V exposure. The results showed that rice roots inhibited the upward movement of V, and root cell walls accumulated 69.85-82.71% of V in roots. Furthermore, hemicellulose-1 (HC-1) in cell walls shared up to 67.72% and 66.95% of the cell-wallbound V in tolerant and sensitive cultivars, respectively. FTIR spectroscopy demonstrated that V stress induced the remolding of cell wall polysaccharides. Under V stress, V-tolerant rice generated up to 19.3% pectin, 40.9% HC-1, and 49.34% HC-2, which were higher than V-sensitive cultivar. The genes encoding UGDH, UGE, and AXS for polysaccharide biosynthesis were higher expressed in V-tolerant rice than V-sensitive rice when exposed to V. The results could provide novel insight for phytoremediation and food security guarantees.

Automated summary

The study found that rice root cell walls have a high binding capacity for vanadium (V), inhibiting its upward movement. Hemicellulose-1 was found to bind most of the V in the cell walls, and V-tolerant rice showed higher levels of cell wall polysaccharides and gene expression under V stress compared to V-sensitive rice.