Unveiling changes of microbial community involved in N and P removal in constructed wetlands with exposing to silver nanoparticles

Constructed wetlands (CWs) are environmentally friendly engineered systems to purify wastewater, with lowcost and easy maintenance. However, it is not clear on responses of functional microbes for nitrogen (N) and phosphorus (P) biotransformation in CWs to silver nanoparticles (Ag NPs). The high throughput sequencings were employed to reveal microbial communities in vertical flow subsurface CWs with stable operation for 120 days. The results indicated that NH4+-N, TN and TP removal of soil layer decreased by 43.56%, 15.7% and 22.7% under stress of Ag NPs. Microbial richness index and compositions were affected, and control wetland enriched Sulfurospirillum, Desulfarculaceae and Flavobacterium whereas CWs exposed to Ag NPs enriched Desulfosporosinus and Desulfurispora from LEfSe analysis. Moreover, after dosing Ag NPs, relative abundances of functional genes amoA and hao for nitrification, nirK and norB for denitrification and ppx and phoA/phoD for phosphorus conversions in upper soil were significantly downregulated. Inhibition on functional bacteria and genes of Ag NPs explained poor removal efficiencies of nitrogen and phosphorus pollutants in CWs. Our findings give an insight into ecological toxicity of Ag NPs on CWs with N and P bioconversions and provide the understanding of response of nitrifiers, denitrifies and PAOs.

Automated summary

The addition of silver nanoparticles in constructed wetlands can decrease nitrogen and phosphorus removal efficiency and alter microbial communities. The relative abundances of functional genes related to nitrogen and phosphorus conversion in the upper soil are significantly decreased after the dosing of silver nanoparticles.