Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 427, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2021.128201
Keywords
Petroleum-degrading bacteria; Bacterial community; 16S rRNA profiling; Oil refining waste; Facultative anaerobes
Categories
Funding
- National Natural Science Foundation of China [32171467, 31570119, 41773080]
- Tianjin Municipal Science and Technology Committee [20JCYBJC01490, 18ZXSZSF00180, 18JCTPJC54400]
- Fundamental Research Funds for the Central Universities [63181220]
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Oil refining waste (ORW) contains complex, hazardous, and refractory components. This study investigates the degradation of ORW using acclimated bacteria from oily sludges and polymer-flooding wastewater. The results show that oxygen and temperature play key roles in ORW degradation, with higher biomass and biodiversity promoting rapid and multi-component degradation. Dominant genera with oxygen-independent sustainable degradation ability are identified as promising bioremediation candidates.
Oil refining waste (ORW) contains complex, hazardous, and refractory components, causing more severe long-term environmental pollution than petroleum. Here, ORW was used to simulate the accelerated domestication of bacteria from oily sludges and polymer-flooding wastewater, and the effects of key factors, oxygen and temperature, on the ORW degradation were evaluated. Bacterial communities acclimated respectively in 30/60 degrees C, aerobic/anaerobic conditions showed differentiated degradation rates of ORW, ranging from 5% to 34%. High-throughput amplicon sequencing and ORW component analysis revealed significant correlation between bacterial diversity/biomass and degradation efficiency/substrate preference. Under mesophilic and oxygen-rich condition, the high biomass and abundant biodiversity with diverse genes and pathways for petroleum hydrocarbons degradation, effectively promoted the rapid and multi-component degradation of ORW. While under harsh conditions, a few dominant genera still contributed to ORW degradation, although the biodiversity was severely restricted. The typical dominant facultative anaerobes Bacillus (up to 99.8% abundance anaembically) and Geobacillus (up to 99.9% abundance aerobically and anaembically) showed oxygen-independent sustainable degradation ability and broad-spectrum of temperature adaptability, making them promising and competitive bioremediation candidates for future application. Our findings provide important strategies for practical bioremediation of varied environments polluted by hazardous ORW.
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