Response of 2,4,6-trichlorophenol-reducing biocathode to burial depth in constructed wetland sediments

Biocathode systems could be used for in-situ bioremediation of chlorophenols (CPs) in constructed wetland (CW) sediments. However, little is known regarding whether or how cathode burial depths affect the dechlorination of CPs in sediments. Here, 2,4,6-trichlorophenol (2,4,6-TCP)-dechlorinating biocathode systems were constructed under a cathode potential of -0.7 V (vs. a saturated calomel electrode, SCE) at three different cathode burial depths (5, 10, and 15 cm). The 2,4,6-TCP removal efficiency and average transformation rate with the biocathode increased by 21.46-36.86% and 14.63-34.88% compared to those in the non-electrode groups. Deeper cathode burial depths enhanced the 2,4,6-TCP dechlorination performance. Furthermore, the oxidationreduction potential (ORP) of the sediment decreased with sediment depth and the applied potential created a more favorable redox environment for the enrichment of functional bacteria. Deeper cathode burial depths also promoted the selective enrichment of electro-active and dechlorinating bacteria (e.g., Bacillus and Dehalobacter, respectively). The biocathode thus served as the carrier, electron source, and regulator of functional bacteria to accelerate the transformation of 2,4,6-TCP (2,4,6-TCP-* 2,4-dichlorophenol-* 4-chlorophenol-* phenol) in sediments. These results offer insights into the effects of cathode burial depth on 2,4,6-TCP dechlorination in sediments from a redox environment and microbial community structure standpoint.

Automated summary

Deeper cathode burial depths were found to enhance the dechlorination of chlorophenols in constructed wetland sediments and promote the enrichment of functional bacteria. The biocathode system can serve as an effective means to accelerate the transformation of chlorophenols.