4.5 Article

Quinoline, with the active site of 8-hydroxyl, efficiently inhibits Micropterus salmoides rhabdovirus (MSRV) infection in vitro and in vivo

Journal

JOURNAL OF FISH DISEASES
Volume 45, Issue 6, Pages 895-905

Publisher

WILEY
DOI: 10.1111/jfd.13615

Keywords

active sites; antiviral activity; Micropterus salmoides rhabdovirus (MSRV); quinoline

Funding

  1. National Natural Science Foundation of China [31972841]

Ask authors/readers for more resources

In this study, the antiviral effects of 22 quinoline derivatives against Micropterus salmoides rhabdovirus (MSRV) were evaluated. 8-hydroxyquinoline was found to effectively inhibit MSRV in vitro and enhance survival rates in MSRV-infected fish in vivo. It also reduced viral loads in the infected fish.
Micropterus salmoides rhabdovirus (MSRV) is an significant pathogen that causes high mortality and related economic losses in bass aquaculture. There is no effective or approved therapy to date. In this study, we evaluated the anti-MSRV effects of 22 quinoline derivatives in grass carp ovary (GCO) cells. Among these compounds, 8-hydroxyquinoline exhibited valid inhibition in decreasing MSRV nucleoprotein gene expression levels of 99.3% with a half-maximal inhibitory concentrations (IC50) value of 4.66 mu M at 48 h. Moreover, 8-hydroxyquinoline significantly enhanced a protective effect in GCO cells by reducing the cytopathic effect (CPE). By comparing the anti-MSRV activity of 22 quinoline derivatives, we found that 8-hydroxyquinoline possessed the efficient active site of 8-hydroxyl and inhibited MSRV infection in vitro. For in vivo studies, 8-hydroxyquinoline via intraperitoneal injection exhibited an antiviral effect in MSRV-infected largemouth bass by substantially enhancing the survival rate by 15.0%. Importantly, the viral loads in the infected largemouth bass notably reduced in the spleen on the third days post-infection. Overall, 8-hydroxyquinoline was considered to be an efficient agent against MSRV in aquaculture.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available