Anaerobic co-digestion of cheese whey and septage: Effect of substrate and inoculum on biogas production

Cheese whey is an industrial waste generated from the cheese processing unit of the dairy industry and requires treatment before its disposal. The present study investigated the possibilities of improving the digestibility from anaerobic digestion of lipid rich dairy by-product, cheese whey using septage as the co-substrate with different inoculum. Biochemical methane potential assays were conducted under mesophilic temperature conditions and results were validated using Modified Gompertz Model. Two sets of BMP tests were done; to assess the individual and combined digestion abilities of septage in anaerobic co-digestion of whey and to assess the ability of 3 inoculum sources (cattle manure, sewage sludge, and acclimatized anaerobic sludge) in the co-digestion process. The results indicated that septage is an excellent co-substrate that has better adaptability with cheese whey and the optimum mix ratio was found as 40:60 (S-CW: S-SP). BMP tests were also conducted with inoculum at S/I ratio of 1 and statistical analysis was performed to study the synergistic effect of both co-digestion and inoculum. The tests revealed that the cattle manure resulted in the highest biogas production (342.22 mL/gVS) at 60% whey fraction. Modified Gompertz model fitted the experimental data well and identified an increase in lag phase times when whey fraction is increased. Comparatively higher lag phase times ranging from 1.98 to 4.35 days were obtained for sewage sludge inoculated samples. The maximum methane production (P-max) was obtained at 60% whey fraction (369.63 +/- 4.05 mL/gVS) at a very short lag time of 0.76 +/- 0.17days for cattle manure inoculated mixture.

Automated summary

The study investigated the improvement of anaerobic digestion of cheese whey waste and found that septage is an excellent co-substrate. The highest biogas production was achieved with cattle manure inoculated mixture at 60% whey fraction.