Journal
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
Volume 69, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jddst.2022.103135
Keywords
siRNA delivery; Polypeptide-based carriers; pH-sensitive delivery systems; Self-assembly; Nanoparticles; Cancer treatment
Categories
Funding
- Russian Foundation of Basic Research (RFBR) [20-33-90181]
- Ministry of Science and Higher Education of the Russian Federation [AAAA-A19-119,021,290,033-1]
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The effective delivery of nucleic acid therapeutics into tumor cells is a crucial aspect of cancer gene therapy. In this study, a series of amphiphilic positively charged biocompatible pH-sensitive polypeptides were synthesized for siRNA delivery. These polypeptides showed self-assembly in aqueous media and formed nanoparticles with favorable characteristics for cellular uptake. The successful down-regulation of gene expression in breast cancer cells demonstrated the potential of these polypeptides as promising non-viral candidates for siRNA delivery.
The effective delivery of nucleic acid therapeutics into tumor cells is of great importance in terms of cancer gene therapy. Successful application of siRNAs is restricted by the requirement to overcome several biological barriers, i.e. cell membrane penetration, endosomal escape, enzymatic degradation in cytosol, etc. Cationic polymers are promising delivery systems due to their biodegradability and effective binding with siRNA. A key characteristic of the particle trafficking into the cell is a noticeable change in pH during this process. This change can be used as a natural stimulus for pH-sensitive polymer particles. Such polymers are able to buffer the environment of a late endosome that leads to an abnormal flow of ions and water followed by endosomal swelling and disrupt and quick escape of the carriers into a cytoplasm. In present work, we synthesized a series of amphiphilic positively charged biocompatible pH-sensitive polypeptides forming polyplexes with nucleic acids. Four amino acids were used to compose the delivery systems, namely (L)-lysine, (L)-glutamic acid, (L)-phenylalanine and (L)-histidine. The number and the ratio of amino acids were varied in order to establish the optimal composition. The characteristics of polypeptides were studied by H-1 NMR spectroscopy, high performance liquid chromatography and static light scattering. All synthesized polypeptides tended to a self-assembly in aqueous media and formed nanoparticles with hydrodynamic diameters ranging from 140 to 360 nm. The formation of polyplexes with short double-stranded oligonucleotides as a physicochemical siRNA model as well as the release of cargo in buffer media mimicking lysosome (pH similar to 5) and cytosol or bloodstream (pH 7.4) were studied. Cellular uptake of the particles was efficient and their cytotoxicity was negligible. Successful RNAi-mediated down-regulation of GFP gene expression was revealed in MDA-MB-231/GFP breast cancer cells. The amphiphilic polypeptides obtained can be considered as promising non-viral candidates for siRNA delivery.
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