Journal
JOURNAL OF DAIRY SCIENCE
Volume 105, Issue 7, Pages 6353-6363Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2022-21874
Keywords
bovine; NGF; progesterone; interferon-stimulated gene
Funding
- National Institute of Food and Agriculture at the USDA Hatch Multi- state Funds (University of California) [1018305]
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This study investigated the effects of nerve growth factor-0 (NGF) from bulls' seminal plasma on progesterone, interferon-stimulated genes (ISG), and pregnancy per artificial insemination (AI) in lactating Holstein dairy cows. The results showed that NGF did not significantly affect progesterone, ISG abundance, or pregnancy rate.
The objectives of this study were to determine the effects of nerve growth factor-0 (NGF), purified from bulls' seminal plasma and administered at the time of artificial insemination (AI), on progesterone post-AI, interferon-stimulated genes (ISG), and pregnancy per AI (P/AI) for lactating Holstein dairy cows enrolled in a timed-AI protocol. We hypothesized that administra-tion of NGF at the time of AI would increase plasma progesterone post-AI, upregulate relative abundance of ISG, and improve P/AI in lactating dairy cows. Hol-stein cows (n = 557) from a single commercial dairy farm were blocked by parity and randomly assigned to receive an intramuscular injection containing 296 mu g of bovine purified NGF at the time of AI, diluted in 2 mL of phosphate-buffered saline (NGF: n = 275), or receive only the 2 mL of phosphate-buffered saline (control: n = 282). Plasma progesterone and corpus luteum size were assessed in a subset of cows (NGF: n = 32; control: n = 36) at d 7, 14, and 19 post-AI. Relative mRNA abundance of ISG (ISG15, MX1, MX2, and RTP4) was assessed in peripheral blood leukocytes on d 19 post-AI. Pregnancy diagnosis was performed at 37 and 65 d post-AI. There was an interaction effect between treatment and parity for plasma progesterone; however, plasma progesterone and ISG did not differ between treatments. There were no effects of NGF for P/AI at 37 d post-AI (NGF = 40.0% vs. control = 41.6%), 65 d post-AI (NGF = 36.0% vs. control = 38.1%), and for pregnancy loss (NGF = 8.4% vs. control = 7.7%). The current study revealed that effects to NGF in lactating Holstein cows were minor and contingent with parity for progesterone, and no improvement in ISG relative abundance and P/AI were observed.
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