Journal
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Volume 137, Issue 1, Pages 214-+Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2015.07.042
Keywords
Nasal vaccine strategy; secretory IgA; reverse transcytosis; microfold cells; nasal-associated lymphoid tissue; protection
Categories
Funding
- Region Rhone-Alpes
- ANRS (France)
- ANRS [2013_1_14255]
- Sidaction [AI19-3-01535]
- Swiss Science Research Foundation [3100-156806]
Ask authors/readers for more resources
Background: Transmission of mucosal pathogens relies on their ability to bind to the surfaces of epithelial cells, to cross this thin barrier, and to gain access to target cells and tissues, leading to systemic infection. This implies that pathogen-specific immunity at mucosal sites is critical for the control of infectious agents using these routes to enter the body. Although mucosal delivery would ensure the best onset of protective immunity, most of the candidate vaccines are administered through the parenteral route. Objective: The present study evaluates the feasibility of delivering the chemically bound p24gag (referred to as p24 in the text) HIV antigen through secretory IgA (SIgA) in nasal mucosae in mice. Results: We show that SIgA interacts specifically with mucosal microfold cells present in the nasal-associated lymphoid tissue. p24-SIgA complexes are quickly taken up in the nasal cavity and selectively engulfed by mucosal dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin-positive dendritic cells. Nasal immunization with p24-SIgA elicits both a strong humoral and cellular immune response against p24 at the systemic and mucosal levels. This ensures effective protection against intranasal challenge with recombinant vaccinia virus encoding p24. Conclusion: This study represents the first example that underscores the remarkable potential of SIgA to serve as a carrier for a protein antigen in a mucosal vaccine approach targeting the nasal environment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available