Journal
JOURNAL OF CLINICAL PERIODONTOLOGY
Volume 49, Issue 10, Pages 1052-1066Publisher
WILEY
DOI: 10.1111/jcpe.13667
Keywords
alveolar bone homeostasis; C-reactive protein; diabetes; periodontal disease; PI3K; AKT pathway
Categories
Funding
- Chongqing Graduate Tutor Team Construction Project [dstd201903]
- National Natural Science Foundation of China [81771082, 31971282, 81800985, 8217096882, 82100986, 81800938]
- Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0851]
Ask authors/readers for more resources
This study explores the role of C-reactive protein (CRP) in periodontitis and diabetes, as well as its mechanism in alveolar bone homeostasis. The results show that in periodontitis and diabetes, CRP can inhibit osteogenesis and promote osteoclastogenesis through the PI3K/AKT signaling pathway, thus disrupting alveolar bone homeostasis.
Aim To explore the role of C-reactive protein (CRP) in periodontitis and diabetes and its mechanism in alveolar bone homeostasis. Materials and Methods In vivo, normal, and Crp knockout (KO) rats were randomly divided into control, diabetes, periodontitis, and diabetes and periodontitis groups, respectively. The diabetes model was established using a high-fat diet combined with streptozotocin injection. The periodontitis model was established by ligature combined with lipopolysaccharide (LPS) injection. Alveolar bones were analysed using micro-computed tomography, histology, and immunohistochemistry. In vitro, human periodontal ligament cells (hPDLCs) were treated with LPS and high glucose. CRP knockdown lentivirus or CRP overexpression adenovirus combined with a PI3K/AKT signalling inhibitor or agonist were used to explore the regulatory mechanism of CRP in osteogenesis and osteoclastogenesis of hPDLCs, as evidenced by alkaline phosphatase staining, Western blot, and quantitative polymerase chain reaction. Results In periodontitis and diabetes, CRP KO decreased the alveolar bone loss and the expression levels of osteoclastogenic markers, while increasing the expression levels of osteogenic markers. CRP constrained osteogenesis while promoting the osteoclastogenesis of hPDLCs via PI3K/AKT signalling under high glucose and pro-inflammatory conditions. Conclusions CRP inhibits osteogenesis and promotes osteoclastogenesis via PI3K/AKT signalling under diabetic and pro-inflammatory conditions, thus perturbing alveolar bone homeostasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available