Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 42, Issue 7, Pages 1224-1235Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0271678X211067455
Keywords
Glymphatic system; histology; in vivo MRI; post-mortem MRI; Virchow-Robin spaces
Categories
Funding
- Austrian Multiple Sklerose Forschungsgesellschaft
- ECTRIMS/MAGNIMS -research fellowship
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The purpose of this study is to evaluate the reproducibility of Virchow-Robin spaces (VRS) detected on in vivo MRI by post-mortem MRI. The results showed that the reproducibility of VRS was higher in the supratentorial white matter and lower in the basal ganglia. This study demonstrated that the majority of VRS are stable throughout death and formalin fixation, highlighting the translational potential of post-mortem VRS studies.
The purpose of our study is to quantify the extent to which Virchow-Robin spaces (VRS) detected on in vivo MRI are reproducible by post-mortem MRI. Double Echo Steady State 3T MRIs were acquired post-mortem in 49 double- and 32 single-hemispheric formalin-fixed brain sections from 12 patients, who underwent conventional diagnostic 1.5 or 3T MRI in median 22 days prior to death (25% to 75%: 12 to 134 days). The overlap of in vivo and post-mortem VRS segmentations was determined accounting for potential confounding factors. The reproducibility of VRS found on in vivo MRI by post-mortem MRI, in the supratentorial white matter was in median 80% (25% to 75%: 60 to 100). A lower reproducibility was present in the basal ganglia, with a median of 47% (25% to 75%: 30 to 50). VRS segmentations were histologically confirmed in one double hemispheric section. Overall, the majority of VRS found on in vivo MRI was stable throughout death and formalin fixation, emphasizing the translational potential of post-mortem VRS studies.
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