Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 42, Issue 10, Pages 1890-1904Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0271678X221104540
Keywords
Glucose metabolism; glutamatergic metabolism; human brain; proton magnetic resonance spectroscopy; ultra-high field strengths
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Funding
- Cancer Prevention and Research Institute of Texas (CPRIT) [679927]
- [RR180056]
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For the first time, labeling effects after oral intake of [1-13C]glucose are observed in the human brain with pure 1H detection at 9.4 T. This study demonstrates that the combination of oral administration of [1-13C]Glc with pure 1H MRS acquisition is a simple and suitable technique to measure metabolic rates in the brain.
For the first time, labeling effects after oral intake of [1-13C]glucose are observed in the human brain with pure 1H detection at 9.4 T. Spectral time series were acquired using a short-TE 1H MRS MC-semiLASER (Metabolite Cycling semi Localization by Adiabatic SElective Refocusing) sequence in two voxels of 5.4 mL in the frontal cortex and the occipital lobe. High-quality time-courses of [4-13C]glutamate, [4-13C]glutamine, [3-13C]glutamate + glutamine, [2-13C] glutamate+glutamine and [3-13C]aspartate for individual volunteers and additionally, group-averaged time-courses of labeled and non-labeled brain glucose could be obtained. Using a one-compartment model, mean metabolic rates were calculated for each voxel position: The mean rate of the TCA-cycle (Vtca) value was determined to be 1.36 and 0.93 mu mol min(-1) g(-1), the mean rate of glutamine synthesis (Vgln) was calculated to be 0.23 and 0.45 mu mol min(-1) g(-1), the mean exchange rate between cytosolic amino acids and mitochondrial Krebs cycle intermediates (Vx) rate was found to be 0.57 and 1.21 mu mol min(-1) g(-1) for the occipital lobe and the frontal cortex, respectively. These values were in agreement with previously reported data. Altogether, it can be shown that this most simple technique combining oral administration of [1-13C]Glc with pure 1H MRS acquisition is suitable to measure metabolic rates.
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