Journal
JOURNAL OF BIOPHOTONICS
Volume 15, Issue 7, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.202100393
Keywords
adipose tissue; diffusion coefficient; ex vivo and in vivo optical clearing; reduced scattering coefficient; refractive index
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Funding
- Decree of the Government of the Russian Federation [220, 075-15-2021-615]
- Olympus Corporation
- Saratov State University
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This study focuses on the development of technology for optical clearing (OC) of abdominal adipose tissue in vivo using hyperosmotic optical clearing agents (OCAs). The results demonstrate that OCAs can effectively clear adipose tissue and induce cell necrosis to a certain extent, both in ex vivo and in vivo experiments.
Optical clearing (OC) of adipose tissue has not been studied enough, although it can be promising in medical applications, including surgery and cosmetology, for example, to visualize blood vessels or increase the permeability of tissues to laser beams. The main objective of this work is to develop technology for OC of abdominal adipose tissue in vivo using hyperosmotic optical clearing agents (OCAs). The maximum OC effect (77%) was observed for ex vivo rat adipose tissue samples exposed to OCA on fructose basis for 90 minutes. For in vivo studies, the maximum effect of OC (65%) was observed when using OCA based on diatrizoic acid and dimethylsulfoxide for 120 minutes. Histological analysis showed that in vivo application of OCAs may induce a limited local necrosis of fat cells. The efficiency of OC correlated with local tissue damage through cell necrosis due to accompanied cell lipolysis
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