Determination of Oxaliplatin and Curcumin in Combination via Micellar HPLC and Its Method Validation

Background A micellar-HPLC method was developed for the determination of oxaliplatin (OHP) and curcumin (CUR) employing a C18 column [4.6 x 250 mm, particle size (dp) = 5 mu m] and diode array detector. Objective A rapid, cost-effective, environmentally friendly, time-efficient, easy-to-handle, and safe method was developed. Methods The conditions were optimized for the estimation of OHP and CUR: 0.15 M sodium dodecyl sulfate (SDS) in 6% (v/v) pentanol buffered to pH 5.0 with a flow rate of 1.0 mL/min, injection volume of 20 mu L, and detection at 325 nm. Different analytical parameters, including linearity, accuracy, precision, robustness, specificity, LOD, and LOQ, were determined in compliance with the International Council on Harmonisation (ICH) guidelines. Results The LOD (S/N = 3) of OHP was 0.004 mu g/mL and for CUR it was 0.005 mu g/mL. The calibration curves for OHP and CUR were linear over the range 0.015-10 mu g/mL (determination coefficient r(2) = 0.9999) and 0.015-10 mu g/mL (r(2) = 0.9994), respectively. Conclusion The drugs were eluted in <12 min and the developed method was applicable for analyzing multiple samples per day. Moreover, it was determined to be robust and was used to quantify OHP and CUR in mice serum/blood. The method could pave the way for quantitative analysis of these drugs during the development of a pharmaceutical preparation for the treatment of colorectal cancer.

Automated summary

A micellar-HPLC method was developed for the determination of oxaliplatin and curcumin, aiming to provide a rapid, cost-effective, environmentally friendly, time-efficient, easy-to-handle, and safe analytical approach. The optimized conditions and compliance with ICH guidelines ensured accuracy and precision in the analysis. The results demonstrated the method's robustness and capability for quantitative analysis of the drugs in mice serum/blood, potentially contributing to the development of pharmaceutical preparations for colorectal cancer treatment.