Potential effect of two Bacillus probiotic strains on performance and fecal microbiota of breeding sows and their piglets

Lay Summary The aim of the present study was to determine if the inclusion of probiotic microorganisms in the mother's diet during gestation and the lactation period is capable of modifying the performance of mothers and piglets and the possible effect on the intestinal health of piglets after separation from the mother. For this, 98 females were distributed in three experimental treatments: a control diet, or the same diet in which one of two probiotic strains to be tested (Bacillus subtilis or Bacillus amyloliquefaciens) were incorporated. The experimental diets were administered during pregnancy and the lactation phase for three consecutive productive cycles. Among the most striking results, it is worth highlighting the impact of probiotic treatments on the reproductive performance of sows. Both supplemented groups showed a higher number of total piglets per sow. Furthermore, sows that received the Bacillus amyloliquefaciens diet showed a significant increase in the number of live-born piglets. Probiotic supplementation also showed effects on the fecal microbiota composition of the mothers and their piglets. Changes in the composition of sow milk were also observed. In summary, results demonstrated the potential benefits of supplementing probiotics, and particularly a strain of Bacillus amyloliquefaciens, to improve prolificacy, modulate the intestinal microbial composition, and improve the performance of piglets during lactation. The effect of long-term administration of two Bacillus strains was tested on 98 breeding sows and their litters allotted into three treatments: a control group (CON); supplemented with 5 x 10(8) cfu/kg B. subtilis - 541 (BSU); or with 5 x 10(8) cfu/kg B. amyloliquefaciens - 516 (BAM). Reproductive and performance variables were recorded over three cycles with 56 dams remaining through the third lactation. Blood and fecal samples were taken longitudinally from 12 sows per treatment on days 8 and 21 of the third lactation and milk samples were taken on day 21. Feces from one piglet per litter was sampled on days 21 and 33 and jejunal gene expression was assessed in two piglets on day 21. Changes in fecal microbiota were assessed by 16S rRNA gene sequencing (Illumina MiSeq) and gene expression by Open-Array technology. Metabolomic responses were analyzed in milk by NMR and Ig-G and Ig-A specific antibodies were determined by ELISA. No significant differences were observed on feed intake, body weight, or fat mobilization of the sows. However, a significant increase in the total number of piglets born was observed in supplemented sows. Although the increase was seen from the first cycle with BAM, improvements were not seen with BSU until the third cycle. BAM also increased the number of born-alive and weaned piglets. NMR analysis showed an impact of BAM on milk composition. No differences were found in milk or blood immunoglobulins. A different structure of the fecal microbiota was found in supplemented sows, with changes across phylum, family, and genus. These changes were greater at day 8, suggesting a relevant role of probiotics establishing a new intestinal balance after labor. Shifts in the microbiota were also seen in the piglets, with a clearer impact post-weaning than in suckling. In this regard, correlations between microbial groups of sows and piglets showed a higher link with weaned (d33) than with suckling pigs (d21), reinforcing the idea of an early maternal carry-over. No changes due to treatment in jejunal gene expression were detected; however, piglet size had a clear impact on different genes. In summary, the addition of both probiotics, and particularly Bacillus amyloliquefaciens, demonstrated potential benefits on the prolificacy of sows. Daily feeding of Bacillus amyloliquefaciens resulted in an increase in the number of weaned piglets. The high correlations between the compositions of the microbiota of sows and their piglets are evidence of maternal imprinting, with effects lasting beyond weaning. The addition of both Bacillus probiotics, without interruption for three consecutive cycles, improved the reproductive performance of the sows in terms of prolificacy. Furthermore, the B. amyloliquefaciens strain also exhibited a significant increase in the number of live piglets born per litter. Probiotic administration for three full cycles appeared to promote a more stable milk composition with B. amyloliquefaciens and was shown to have a clear impact on the gut microbial ecosystem of sows.

Automated summary

The aim of this study was to determine the effects of probiotic supplementation during gestation and lactation on the performance of sows and piglets, as well as the intestinal health of piglets after separation from the mother. Results showed that probiotic supplementation improved the reproductive performance of sows and had an impact on the fecal microbiota composition of both mothers and piglets.