Design, Synthesis, Anti-Tomato Spotted Wilt Virus Activity, and Mechanism of Action of Thienopyrimidine-Containing Dithioacetal Derivatives

Currently, there is insufficient viricide to effectively control tomato spotted wilt virus (TSWV). To address thispending issue, a series of thienopyrimidine-containing dithioacetal derivatives were prepared and tested for their anti-TSWVactivities. A subsequent three-dimensional quantitative structure-activity relationship was constructed to indicate the developmentof optimal compound35. The obtained compound35had excellent anti-TSWV curative, protective, and inactivating activities (63.0,56.6, and 74.1%, respectively), and the EC50values of protective and inactivating activities of compound35were 252.8 and 113.5mg/L, respectively, better than those of ningnanmycin (284.8 and 144.7 mg/L) and xiangcaoliusuobingmi (624.9 and 300.0 mg/L).In addition, the anti-TSWV activity of compound35was associated with defense-related enzyme activities, enhancedphotosynthesis, and reduced stress response, thereby enhancing disease resistance

Automated summary

This study prepared and tested a series of compounds with anti-TSWV activities, among which compound35 showed promising curative and protective effects. The anti-TSWV activity of compound35 was associated with defense-related enzyme activities, enhanced photosynthesis, and reduced stress response.