Biocontrol Effect of Bacillus subtilis YPS-32 on Potato Common Scab and Its Complete Genome Sequence Analysis

Potato common scab is caused by Streptomyces, which resides in soil and has become a serious disease in potato planting areas worldwide. In this study, we obtained a Bacillus subtilis YPS-32 strain by natural screening, and atmospheric and roomtemperature plasma (ARTP) mutagenesis and field trial results showed that B. subtilis YPS-32 has a control efficacy of 83.70% against potato common scab. The complete genome of B. subtilis YPS-32 was sequenced, and multiple genes related to the synthesis of antibiotics and plant growth promoters were detected. Based on the genomic information for B. subtilis YPS-32, the sfp geneinactivated (related to the synthesis of secondary metabolites) mutant strain B. subtilis YPS-32 Delta sfp was constructed. Analysis of crude extract metabolites using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) techniques revealed that strain YPS-32 encodes antagonists, such as surfactin and fengycin, which have antimicrobial effects. This study clarifies the mode of action by which B. subtilis YPS-32 antagonizes Streptomyces scabies and provides a reference for further research on antibacterial genes in the future.

Automated summary

In this study, a Bacillus subtilis YPS-32 strain was obtained and found to have a control efficacy of 83.70% against potato common scab. Analysis of the strain's genome revealed multiple genes related to the synthesis of antibiotics and plant growth promoters. The study also identified antagonists produced by the strain that have antimicrobial effects. The findings provide insights into the mode of action of B. subtilis YPS-32 against Streptomyces scabies and serve as a reference for future research on antibacterial genes.