Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 70, Issue 23, Pages 7077-7084Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c01246
Keywords
human milk; bioactive peptide; transepithelial; Caco-2 cell; digestion
Funding
- National Institutes of Health [5TL1TR002371-03, 1S10OD020111-01]
- USDA National Institute of Food and Agriculture [2018-67017-27521]
- USDA multistate workgroup [W4002]
- Gerber Foundation [2017-1586]
Ask authors/readers for more resources
The study showed that certain milk peptides can cross the intestinal cell monolayer and reach the bloodstream to exert bioactivities.
Human milk-protein-derived peptides exhibit an array of bioactivities. Certain bioactivities cannot be exerted unless the peptides are absorbed across the gastrointestinal lumen into the bloodstream. The purpose of study was to determine which peptides derived from in vitro digestion of human milk could cross human intestinal Caco-2 cell monolayers. Our results showed that the numbers of peptides absorbed by the Caco-2 cell monolayer were different at different concentrations (44 peptides out of 169 peptides detected at 10 mu g/mL, 124 peptides out of 204 peptides detected at 100 mu g/mL, and 175 peptides out of 236 peptides detected at 1000 mu g/mL). Four peptides (NLHLPLP (beta-casein [138-144]), PLAPVHNPI (beta-casein [216-224]), PLMQQVPQPIPQ (beta-casein [148-159]), and FDPQIPK (beta-casein [126-132])) crossed to the basolateral chamber of the Caco-2 monolayer incubated with peptides at all three concentrations. Among the peptides identified in the basolateral chambers, three peptides (NLHLPLP (beta-casein [138-144]), LENLHLPLP (beta-casein [136-144]), and QVVPYPQ (beta-casein [182-188])) are known ACE-inhibitors; one peptide (LLNQELLLNPTHQIYPV (beta-casein [197-213])) is antimicrobial, and another peptide (QVVPYPQ (beta-casein [182-188])) has antioxidant activity. These findings indicate that specific milk peptides may be able to reach the bloodstream and exert bioactivity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available