Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 70, Issue 16, Pages 5015-5025Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c01034
Keywords
Schisandra chinensis fruit; anti-inflammatory; allergic asthma; UPLC-Q/TOF-MS; isolation and identification; network pharmacology
Funding
- National Natural Science Foundation of China [81872965]
- CAMS Innovation Fund for Medical Sciences [2021-I2M-1-031]
- Fundamental Research Funds for the Central Universities [3332021049]
Ask authors/readers for more resources
The study found that PET2, a subfraction of the extract from Schisandra chinensis fruit, exhibited significant anti-inflammatory effects and potential anti-asthma properties. Six compounds from PET2 were identified to have good anti-inflammatory properties, possibly through the involvement of specific signaling pathways.
Schisandra chinensis fruit is a widely edible and medicinal resource, whose extract had a good inhibitory effect on airway inflammation in asthmatic mice. However, the main active components remain unknown. In this work, we found that PET2, a subfraction of its ethanolic extract petroleum ether, displayed significant anti-inflammatory effects in interleukin (IL)-4/tumor necrosis factor (TNF)-alpha-stimulated BEAS-2B cells. Meanwhile, in the ovalbumin (OVA)-induced allergic asthma mice model, PET2 (200 and 400 mg/kg) had significant effects on attenuating airway inflammatory cell infiltration and reducing serum Th2-related cytokines. Further studies led to the isolation and identification of 14 compounds, guided by ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based rapid characterization of chemical constituents. Combining network pharmacology analysis and in vitro experiments, we found that six compounds from PET2 had good anti-inflammatory properties. The potential mechanism may be involved in Fc epsilon RI, T cell receptor, and Jak-STAT signaling pathways. This study clarified the anti-inflammatory properties of the main active fraction and active compounds of S. chinensis fruit and provided a theoretical basis for its anti-asthma scientific utilization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available