Mameliella sediminis sp. nov., a novel polyhydroxyalkanoate-accumulating bacterium

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterium, capable of producing poly-beta-hydroxyalkanoate, designated DP3N28-2(T), was isolated from the sediment collected from Daya Bay, Guangdong, PR China. Optimal growth occurred at 37-40 degrees C, pH 6.0 and in the presence of 4% NaCl. The 16S rRNA gene sequences analysis revealed that DP3N28-2(T) showed highest similarities with Mameliella alba DSM 23384(T) (98.3%), Antarctobacter jejuensis 13-2-B6(T) (97.2%), Antarctobacter helio-thermus El-219(T) (96.8%), Maliponia aquimaris MM-10(T) (96.7%), Ponticoccus litoralis CL-GR66(T) (96.4%) and Aquicoccus porphyridii L1 8-17(T) (96.1%). The predominant fatty acids (>10%) were summed feature 8 (C-18:1 omega 6c and/or C-18:1 omega 7c; 72.1%) and C-1(6:)0 (11.0%). The polar lipids contain phosphatidylethanolamine, phosphatidylmonomethylethanolamine. phosphatidylglycerol, one aminophosphlipid, one phospholipid and three unidentified lipids. The respiratory quinone was Q-10. The DNA G+C content was 63.0 mol% (data from the genome sequence). The estimated genome size was 5.12 Mb. The average nucleotide identity values between the DP3N28-2(T) genome and the genome of M. alba was 81.1 %, while the digital DNA-DNA hybridization value was 23.4%. The phenotypic, genotypic and chemotaxonomic differences between DP3N28-2(T) and its phylogenetic relatives indicates that DP3N28-2(T) should be regarded as representing a novel species of the genus Mameliella, for which the name Mameliella sediminis sp. nov. is proposed. The type strain is DP3N28-2(T) (=MCCC 1K06218(T)=KCTC 82804(T)).

Automated summary

In this study, a poly-beta-hydroxyalkanoate producing bacterium DP3N28-2(T) was isolated from the sediment of Daya Bay, Guangdong, PR China. Based on the morphological, physiological, biochemical, and genetic analyses, it was identified as a novel species of the genus Mameliella, for which the name Mameliella sediminis sp. nov. is proposed.