Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 618, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.ijpharm.2022.121630
Keywords
Animal studies; Dosing technique; Gastrointestinal transit; Paracetamol; X-ray imaging; Microcontainers
Categories
Funding
- Danish National Research Foundation [DNRF122]
- Villum Fonden [9301]
- Center for intelligent drug delivery and sensing using microcontainers and nanomechanics (IDUN)
Ask authors/readers for more resources
This study investigated the impact of three oral gavage techniques on gastrointestinal transit and drug absorption. The results showed that piston-dosing was the superior technique for promoting drug absorption.
Oral gavage is the most common way to administer drug formulations orally to rats. Yet, the technique applied and its influence on gastrointestinal (GI) transit receive little attention. This study aims to investigate the impact of three oral gavage techniques on GI transit and drug absorption utilizing microcontainers (MCs). The MCs were filled with paracetamol and BaSO4 (1:1 w/w ratio), coated with Eudragit S100, and filled into size-9 gelatin capsules. An in vitro study confirmed the intactness of the coating, and the capsules were administered to rats with air, water, or a piston. X-ray imaging determined the locations of the MCs, and the corresponding plasma concentration of paracetamol established a correlation with the location. The fastest GI transit occurred with air dosing, while water-dosing caused delayed gastric emptying for 3 h with non-quantifiable paracetamol absorption. Piston-dosed MCs were retained in the stomach for up to 1 h, though for 3 h in one rat. Air-dosing caused discomfort and stress in rats, thus limiting its ethical and physiological relevance. Water-dosing confined its use due to delayed gastric emptying. In conclusion, the oral gavage technique affected the GI transit of MCs and, consequently, drug absorption. Piston-dosing appeared to be the superior dosing technique.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available