Poly(DL-lactic acid) scaffolds as a bone targeting platform for the co-delivery of antimicrobial agents against S. aureus-C.albicans mixed biofilms

New strategies for the treatment of polymicrobial bone infections are required. In this study, the co-delivery of two antimicrobials by poly(D,L-lactic acid) (PDLLA) scaffolds was investigated in a polymicrobial biofilm model. PDLLA scaffolds were prepared by solvent casting/particulate leaching methodology, incorporating minocycline and voriconazole as clinically relevant antimicrobial agents. The scaffolds presented a sponge-like appearance, suitable to support cell proliferation and drug release. Single- and dual-species biofilm models of Staphylococcus aureus and Candida albicans were developed and characterized. S. aureus presented a higher ability to form singlespecies biofilms, compared to C. albicans. Minocycline and voriconazole-loaded PDLLA scaffolds showed activity against S. aureus and C. albicans single- and dual-biofilms. Ultimately, the cytocompatibility/functional activity of PDLLA scaffolds observed in human MG-63 osteosarcoma cells unveil their potential as a next-generation codelivery system for antimicrobial therapy in bone infections.

Automated summary

In this study, the co-delivery of two antimicrobials using poly(D,L-lactic acid) (PDLLA) scaffolds was investigated in a polymicrobial biofilm model. The PDLLA scaffolds showed a sponge-like appearance and were capable of supporting cell proliferation and drug release. The loaded scaffolds demonstrated activity against Staphylococcus aureus and Candida albicans biofilms. Thus, the study revealed the potential of these scaffolds as a next-generation co-delivery system for antimicrobial therapy in bone infections.