Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/ijms23095130
Keywords
tomato; S; cheesmaniae; salt tolerance; rootstock breeding; yield; fruit quality; K+ and Na+ homeostasis; long-distance transport
Funding
- Spanish Ministerio de Ciencia e Innovacion/Agencia Estatal de Investigacion [AGL2017-82452-C2-1R, AGL2017-82452-C2-2R, MCIN/AEI/10.13039/501100011033]
- European Union [101000716]
- FEDER Una manera de hacer Europa [JAEINT_19_00566]
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This study used RNAi technology to reduce the expression level of Na+ transporters in tomato. By studying different rootstock/scion combinations, it was found that silencing the SgHKT1;1 gene in the rootstock can improve salt tolerance and fruit quality of tomato varieties carrying the wild type SgHKT1;2 allele.
Salt tolerance is a target trait in plant science and tomato breeding programs. Wild tomato accessions have been often explored for this purpose. Since shoot Na+/K+ is a key component of salt tolerance, RNAi-mediated knockdown isogenic lines obtained for Solanum galapagense alleles encoding both class I Na+ transporters HKT1;1 and HKT1;2 were used to investigate the silencing effects on the Na and K contents of the xylem sap, and source and sink organs of the scion, and their contribution to salt tolerance in all 16 rootstock/scion combinations of non-silenced and silenced lines, under two salinity treatments. The results show that SgHKT1;1 is operating differently from SgHKT1;2 regarding Na circulation in the tomato vascular system under salinity. A model was built to show that using silenced SgHKT1;1 line as rootstock would improve salt tolerance and fruit quality of varieties carrying the wild type SgHKT1;2 allele. Moreover, this increasing effect on both yield and fruit soluble solids content of silencing SgHKT1;1 could explain that a low expressing HKT1;1 variant was fixed in S. lycopersicum during domestication, and the paradox of increasing agronomic salt tolerance through silencing the HKT1;1 allele from S. galapagense, a salt adapted species.
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