Theophylline Extracted from Fu Brick Tea Affects the Metabolism of Preadipocytes and Body Fat in Mice as a Pancreatic Lipase Inhibitor

The dramatic increase in obesity is putting people under increasing pressure. Lipase inhibitors, as a kind of effective anti-obesity drug, have attracted more and more researchers' attention in recent years because of their advantages of acting on the intestinal tract and having no side effects on the central nervous system. In this study, lipase inhibitor Fu Brick Theophylline (FBT) was screened based on enzyme molecular dynamics, and the inhibition mechanism of lipase inhibitors on obesity was analyzed and discussed at the cellular level and animal model level. We found that FBT had high inhibition effects of lipase with an IC50 of 1.02~0.03 mu g/mL. Firstly, the laboratory used 3T3-L1 proadipocytes as models, flow cytometry was used to detect the effects of FBT on the cycle, apoptosis and intracellular ROS activity of proadipocytes. To study the contents of triglyceride, total cholesterol, related metabolites and related gene and protein expression in adipocytes. The results showed that FBT could reduce ROS production and inflammatory factor mRNA expression during cell differentiation. Secondly, by establishing the animal model of high-fat feed ob nutritional obese mice, the morphological observation and gene expression analysis of body weight, fat rate, adipocyte and hepatocyte metabolism of FBT obese mice were further discussed. It was proven that FBT can effectively reduce the degree of fatty liver, prevent liver fibrosis and fat accumulation, and improve the damage of mitochondrial membrane structure. This study provides a theoretical basis for the screening and clinical treatment of lipase inhibitors.

Automated summary

The dramatic increase in obesity has led to a growing interest in lipase inhibitors as effective anti-obesity drugs. In this study, a lipase inhibitor called Fu Brick Theophylline (FBT) was screened using enzyme molecular dynamics. The study analyzed and discussed the mechanism of lipase inhibitors in combating obesity at the cellular and animal model levels. The results showed that FBT had a strong inhibitory effect on lipase and could reduce the generation of reactive oxygen species (ROS) and expression of inflammatory factors during cell differentiation. Animal models further confirmed that FBT effectively reduced the degree of fatty liver and improved mitochondrial membrane damage. This study provides a theoretical basis for the screening and clinical treatment of lipase inhibitors.