Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms23073719
Keywords
root; shotgun proteomics; Mn deficiency; Fe deficiency; tomato; 2-DE
Funding
- Spanish Research Agency [PID2020-115856RB-100, MCIN/AEI/ 10.13039/501100011033]
- Aragon Government [A09-20R]
- JSPS KAKENHI Grant [24-7373, 22120003, 24370018]
- FPI-MINECO
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Iron and manganese are essential elements for plants, but they compete for the same uptake transporters and show conflicting interactions at the regulatory level. Proteomic techniques were used to study the proteome profiles of tomato roots grown under iron or manganese deficiency. The results showed that both deficiencies led to common cell wall remodelling, but differed greatly in terms of oxidative stress, coumarin production, protein, nitrogen, and energy metabolism.
Iron (Fe) and manganese (Mn) are two essential elements for plants that compete for the same uptake transporters and show conflicting interactions at the regulatory level. In order to understand the differential response to both metal deficiencies in plants, two proteomic techniques (two-dimensional gel electrophoresis and label-free shotgun) were used to study the proteome profiles of roots from tomato plants grown under Fe or Mn deficiency. A total of 119 proteins changing in relative abundance were confidently quantified and identified, including 35 and 91 in the cases of Fe deficiency and Mn deficiency, respectively, with 7 of them changing in both deficiencies. The identified proteins were categorized according to function, and GO-enrichment analysis was performed. Data showed that both deficiencies provoked a common and intense cell wall remodelling. However, the response observed for Fe and Mn deficiencies differed greatly in relation to oxidative stress, coumarin production, protein, nitrogen, and energy metabolism.
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