Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 6, Pages 1326-1334Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b04966
Keywords
gallic acid; ellagic acid; hydrolyzable tannins; gallotannins; ellagitannins; methanolysis; methyl gallate; UHPLC; electrospray; MS/MS
Funding
- PepsiCo
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Hydrolyzable tannins are measured routinely during the characterization of food and beverage samples. Most methods for the determination of hydrolyzable tannins use hydrolysis or methanolysis to convert complex tannins to small molecules (gallic acid, methyl gallate, and ellagic acid) for quantification by HPLC-UV. Often unrecognized, analytical limitations and variability inherent in these approaches for the measurement of hydrolyzable tannins include the variable mass fraction (0-0.90) that is released as analyte, contributions of sources other than tannins to hydrolyzable gallate (can exceed >10 wt %/wt), the measurement of both free and total analyte, and lack of controls to account for degradation. An accurate, specific, sensitive, and higher-throughput approach for the determination of hydrolyzable gallate based on ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) that overcomes these limitations was developed.
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