Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/ijms23105645
Keywords
Arylquin 1; colon cancer; tumor progression; apoptosis
Funding
- Ministry of Science and Technology [MOST 107-2320-B-037-018, MOST 110-2314-B-037-075-MY2]
- Kaohsiung Medical University Chung-Ho Memorial Hospital [KMUH107-7R87, KMUH108-8M66, KMUH109-9M78, KMUH110-0R72, KMUHDK(B)110005-2]
- Kaohsiung Medical University [KMU-Q108003]
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Arylquin 1 exhibits anticancer effects in colorectal cancer cells by inhibiting cell proliferation, migration, and invasion, and inducing apoptosis. It increases the phosphorylation levels of ERK, JNK, and p38, which are related to key signaling proteins.
Colorectal cancer (CRC) is one of the most common gastrointestinal cancers worldwide. Current therapeutic strategies mainly involve surgery and chemoradiotherapy; however, novel antitumor compounds are needed to avoid drug resistance in CRC, as well as the severe side effects of current treatments. In this study, we investigated the anticancer effects and underlying mechanisms of Arylquin 1 in CRC. The MTT assay was used to detect the viability of SW620 and HCT116 cancer cells treated with Arylquin 1 in a dose-dependent manner in vitro. Further, wound-healing and transwell migration assays were used to evaluate the migration and invasion abilities of cultured cells, and Annexin V was used to detect apoptotic cells. Additionally, Western blot was used to identify the expression levels of N-cadherin, caspase-3, cyclin D1, p-extracellular signal-regulated kinase (ERK), p-c-JUN N-terminal kinase (JNK), and phospho-p38, related to key signaling proteins, after administration of Arylquin 1. Xenograft experiments further confirmed the effects of Arylquin 1 on CRC cells in vivo. Arylquin 1 exhibited a dose-dependent reduction in cell viability in cultured CRC cells. It also inhibited cell proliferation, migration, and invasion, and induced apoptosis. Mechanistic analysis demonstrated that Arylquin 1 increased phosphorylation levels of ERK, JNK, and p38. In a mouse xenograft model, Arylquin 1 treatment diminished the growth of colon tumors after injection of cultured cancer cells. Arylquin 1 may have potential anticancer effects and translational significance in the treatment of CRC.
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