Biodegradation and Metabolic Pathway of 17β-Estradiol by Rhodococcus sp. ED55

Endocrine disrupting compounds (EDCs) in the environment are considered a motif of concern, due to the widespread occurrence and potential adverse ecological and human health effects. The natural estrogen, 17 beta-estradiol (E2), is frequently detected in receiving water bodies after not being efficiently removed in conventional wastewater treatment plants (WWTPs), promoting a negative impact for both the aquatic ecosystem and human health. In this study, the biodegradation of E2 by Rhodococcus sp. ED55, a bacterial strain isolated from sediments of a discharge point of WWTP in Coloane, Macau, was investigated. Rhodococcus sp. ED55 was able to completely degrade 5 mg/L of E2 in 4 h in a synthetic medium. A similar degradation pattern was observed when the bacterial strain was used in wastewater collected from a WWTP, where a significant improvement in the degradation of the compound occurred. The detection and identification of 17 metabolites was achieved by means of UPLC/ESI/HRMS, which proposed a degradation pathway of E2. The acute test with luminescent marine bacterium Aliivibrio fischeri revealed the elimination of the toxicity of the treated effluent and the standardized yeast estrogenic (S-YES) assay with the recombinant strain of Saccharomyces cerevisiae revealed a decrease in the estrogenic activity of wastewater samples after biodegradation.

Automated summary

The bacterial strain Rhodococcus sp. ED55 isolated from sediments of a discharge point of a wastewater treatment plant in Macau was found to effectively degrade 17 beta-estradiol (E2) and its metabolites, with the potential to eliminate toxicity in treated effluent and reduce estrogenic activity in wastewater samples. The results suggest that Rhodococcus sp. ED55 shows promise as a bioremediation agent for E2-contaminated environments.