Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/ijms23063170
Keywords
mesenchymal stem; stromal cells; post mortem donors; knee; peri-knee tissues; bone and bone marrow; synovium; periosteum; in vitro analysis; human skeletal stem cell markers
Funding
- Slovenian Research Agency [J3-1749]
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This study aimed to identify the optimal tissue source for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties and to determine the influence of post mortem time on these cells. The results showed that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.
Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of CD146 in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.
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