Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/ijms23052691
Keywords
aging; ceramide; ceramide-1-phosphate; diesel particulate extract; matrix metalloprotease; skin
Funding
- Main Research Program of the Korea Food Research Institute (KFRI) funded by the Ministry of Science and ICT [E0210600-01]
- National Research Council of Science & Technology (NST), Republic of Korea [E0210600-01] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Both intrinsic and extrinsic factors, such as an individual's body clock and air pollutants, accelerate premature aging. This study demonstrates that diesel particulate extract (DPE) increases cell senescence and activates matrix metalloprotease (MMP)-1 and MMP-3, leading to collagen degradation in human dermal fibroblasts. DPE activates NADPH oxidase and increases ceramide production, which in turn activates the arachidonate cascade and promotes MMP activity.
Both intrinsic (i.e., an individual's body clock) and extrinsic factors (i.e., air pollutants and ultraviolet irradiation) accelerate premature aging. Epidemiological studies have shown a correlation between pollutant levels and aging skin symptoms. Diesel particle matter in particular leads to some diseases, including in the skin. Our recent study demonstrates that diesel particulate extract (DPE) increases apoptosis via increases in an anti-mitogenic/pro-apoptotic lipid mediator, ceramide in epidermal keratinocytes. Here, we investigated whether and how DPE accelerates premature skin aging using cultured normal human dermal fibroblasts (HDF). We first demonstrated that DPE increases cell senescence marker beta-galactosidase activity in HDF. We then found increases in mRNA and protein levels, along with activity of matrix metalloprotease (MMP)-1 and MMP-3, which are associated with skin aging following DPE exposure. We confirmed increases in collagen degradation in HDF treated with DPE. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) is activated by DPE and results in increased ceramide production by sphingomyelinase activation in HDF. We identified that ceramide-1-phosphate (C1P) (produced from ceramide by ceramide kinase activation) activates MMP-1 and MMP-3 through activation of arachidonate cascade, followed by STAT 1- and STAT 3-dependent transcriptional activation.
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