Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 6, Pages 1377-1384Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05998
Keywords
Fusarium mycotoxins; stable isotope dilution assay; LC-MS/MS; quantitative polymerase chain reaction; barley; malting process
Funding
- Faculty Graduate Center Weihenstephan of TUM Graduate School at Technische Universitat Munchen, Germany
- Forschungskreis der Ernahrungsindustrie e.V. (FEI, Bonn)
- AiF
- German Federal Ministry of Economic Affairs and Energy (AiF-Project) [17221 N]
- Wissenschaftsforderung der Deutschen Brauwirtschaft e.V.
- Wissenschaftliche Station fur Brauerei in Munchen e.V.
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Little is known about the fate of Fusarium mycotoxins during the barley malting process. To determine the fungal DNA. and mycotoxin concentrations during malting, we used barley grain harvested from field plots that we had inoculated with Fusarium species that produce type A or type B trichothecenes or enniatins. Using a recently developed multimycotoxin liquid chromatography tandem mass stable isotope dilution method, we identified Fusarium-species-specific behaviors of mycotoxins in grain and malt extracts and compared toxin concentrations to amounts of fungal DNA in the same samples. In particular, the type B trichothecenes and Fusarium culmorum DNA contents were increased dramatically up to 5400% after kilning. By contrast, the concentrations of type A trichothecenes and Fusarium sporotrichioides DNA decreased during the malting process. These data suggest that specific Fusarium species that contaminate the raw grain material might have different impacts on malt quality.
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