Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 39, Pages 7335-7341Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b02719
Keywords
Brewer's spent yeast; gastrointestinal digestion; transepithelial transport; ACE-inhibitory activity; antioxidant activity
Funding
- FCT/MEC through national funds [UID/QUI/50006/2013-POCI/01/0145/FERDER/007265, UID/MULTI/04378/2013-POCI/01/0145/FERDER/007728]
- FEDER
- Fundacao Ciencia e Tecnologia [SFRH/BD/81845/2011]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/81845/2011] Funding Source: FCT
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Brewer's spent yeast (BSY) autolysates may have potential applications as food ingredients or nutraceuticals due to their antioxidant and ACE-inhibitory activities. The impact of simulated gastrointestinal (GI) digestion, the interaction with intracellular sources of oxidative stress, the intestinal cell permeability of BSY peptides, and the antioxidant and ACE-inhibitory activities of BSY permeates were assayed. Gastrointestinal digestion of BSY autolysates enhanced antioxidant and ACE-inhibitory activities as measured in vitro. No cytotoxic effects were observed on Caco-2 cells after exposure to the digested BSY autolysates within a concentration range of 0.5 to 3.0 mg of peptides/mL. A protective role to induced oxidative stress was observed. The transepithelial transport assays indicate high apparent permeability coefficient (P-app) values for BSY peptides across Caco-2/HT29-MTX cell monolayer (14.5-26.1 X 10(-6) cm/s) and for Caco-2 cell monolayer model (12.4-20.8 X 10(-6) cm/s), while the antioxidant and ACE-inhibitory activities found in flux material from the basolateral side suggest transepithelial absorption of bioactive compounds.
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