4.7 Article

Charged Residue Implantation Improves the Affinity of a Cross-Reactive Dengue Virus Antibody

Journal

Publisher

MDPI
DOI: 10.3390/ijms23084197

Keywords

cross-reactive antibody; dengue virus; affinity maturation; charge complementarity; molecular dynamics simulation

Funding

  1. National Natural Science Foundation of China [81902059, 32071448]
  2. Major Projects of Guangdong Education Department for Foundation Research and Applied Research [2020A1515011170]
  3. Sun Yat-sen University's Research Grant [19ykzd28]

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This study demonstrated the utility of tweaking antibody-antigen charge complementarity for affinity maturation and emphasized the complexity of improving antibody affinity toward multiple antigens. By analyzing the structure of a specific antibody-antigen complex and designing mutations based on complementary charges, the researchers were able to enhance the affinity of the antibody towards different serotypes of dengue virus. The results showed that the mutations improved affinity through different mechanisms and had additive effects on affinity improvement.
Dengue virus (DENV) has four serotypes that complicate vaccine development. Envelope protein domain III (EDIII) of DENV is a promising target for therapeutic antibody development. One EDIII-specific antibody, dubbed 1A1D-2, cross-reacts with DENV 1, 2, and 3 but not 4. To improve the affinity of 1A1D-2, in this study, we analyzed the previously solved structure of 1A1D-2-DENV2 EDIII complex. Mutations were designed, including A54E and Y105R in the heavy chain, with charges complementary to the epitope. Molecular dynamics simulation was then used to validate the formation of predicted salt bridges. Interestingly, a surface plasmon resonance experiment showed that both mutations increased affinities of 1A1D-2 toward EDIII of DENV1, 2, and 3 regardless of their sequence variation. Results also revealed that A54E improved affinities through both a faster association and slower dissociation, whereas Y105R improved affinities through a slower dissociation. Further simulation suggested that the same mutants interacted with different residues in different serotypes. Remarkably, combination of the two mutations additively improved 1A1D-2 affinity by 8, 36, and 13-fold toward DENV1, 2, and 3, respectively. In summary, this study demonstrated the utility of tweaking antibody-antigen charge complementarity for affinity maturation and emphasized the complexity of improving antibody affinity toward multiple antigens.

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