Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 44, Pages 8305-8314Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b03095
Keywords
Beta vulgaris L.; chemical composition; polyphenol oxidase activity; phenolic compounds; greenhouse study
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Effects of the developmental stage (e.g., young, mature, or senescent) of leaves on their chemical composition have been described in the literature. This study focuses on the variation in chemical composition and quantity and quality of proteins extracted from leaves due to variation in plant age (i.e., harvesting time), using leaves from sugar beets grown in a field (Rhino, Arrival) and in a greenhouse (Isabella). Within the same variety (Rhino, field; Arrival, field; Isabella, greenhouse) the protein content was similar for leaves from young and old plants (22 +/- 1, 16 +/- 1, and 10 +/- 3% w/w db, respectively). Variation in final protein isolation yield was mostly due to variation in nitrogen extractability (28-56%), although no consistent correlation with plant age was found. A significant effect of plant age was observed on the quality (color) of the extracted protein, that is, brown (indicative of polyphenol oxidase activity) and yellow for extracts from old and young plants, respectively.
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