Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 5, Pages 1158-1163Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05729
Keywords
SIDA; HS-SPME/GC-MS; skatole; microsomes; metabolism
Funding
- DFG (Deutsche Forschungsgemeinschaft) [WU 322/5-1]
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Skatole metabolites have been considered as putative contributors to boar taint. Recently, 2-aminoacetophenone, a volatile phase I skatole metabolite, was identified in back fat samples from boars of Pietrain X Baden-Wurttemberg hybrid type. This paper addresses the question of the physiological origin of the observed 2-aminoacetophenone in these pigs. Microsomal fractions from nine boars were isolated, and formation of skatole metabolites was subsequently analyzed by stable-isotope dilution analysis (SIDA) using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS). Significant breed-related differences in phase I skatole metabolism were observed, explaining the high levels of 2aminoacetophenone in Pietrain x Baden-Wurttemberg hybrid type boars.
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