Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 4, Pages 821-830Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05577
Keywords
obesity; adipogenesis; bisdemethoxycurcumin (BDMC); mitotic clonal expansion (MCE); high-fat diet
Funding
- National Taiwan University [NTU-104R7777]
- Ministry of Science and Technology [101-2628-B-022-001-MY4, 102-2628-B-002-053-MY3]
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Obesity is caused by excessive accumulation of body fat and is closely related to complex metabolic diseases. Adipogenesis is a key process that is required in adipocyte hypertrophy in the development of obesity. Curcumin (Cur) has been reported to inhibit adipocyte differentiation, but the inhibitory effects of other curcuminoids present in turmeric, such as demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), on adipogenesis have not been investigated. Here, we investigated the effects of curcuminoids on adipogenesis and the molecular mechanisms of adipocyte differentiation. Among three curcuminoids, BDMC was the most effective suppressor of lipid accumulation in adipocytes. BDMC suppressed adipogenesis in the early stage primarily through attenuation of mitotic dong expansion (MCE). In BDMC-treated preadipocytes, cell cycle arrest at the G(0)/G(1) phase was found after initiation of adipogenesis and was accompanied by downregulation of cyclin A, cydin B, p21, and mitogen-activated protein kinase (MAPK) signaling. The protein levels of the adipogenic transcription factors peroxisome proliferator-activated receptor (PPAR)gamma and CCAAT/enhancer-binding proteins (C/EBP)alpha were also reduced by BDMC treatment. Furthermore, 0.5% dietary BDMC (w/w) significantly lowered body weight gain and adipose tissue mass in high-fat diet (HFD)-fed mite. The results of H&E staining showed that dietary BDMC reduced hypertrophy in adipocytes. These results demonstrate for the first time that BDMC suppressed adipogenesis in 3T3-L1 adipocytes and prevented HFD-induced obesity. Our results suggest that BDMC has the potential to prevent obesity.
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